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omniscript reverse transcriptase kit  (Qiagen)


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    Structured Review

    Qiagen omniscript reverse transcriptase kit
    Omniscript Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 6786 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/omniscript+reverse+transcriptase+kit/Reverse+transcriptase/pm41928441-371-18-22
    Average 97 stars, based on 6786 article reviews
    omniscript reverse transcriptase kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Trematode infection buffers heat stress in blue mussels Mytilus edulis : The role of heat shock proteins
    Article Snippet: Qiagen TissueLyser was used to homogenise the samples, after which RNA extraction was completed using a MicroEluete® Total RNA Kit I (Omega Bio‐Tek), according to the manufacturer's instructions. .. A QubitMT RNA BR Assay Kit and a Qubit® 2.0 Fluorometer were used to quantify the total amount of RNA. cDNA was synthesised from 1000 ng of total RNA from each sample using the Omniscript reverse transcriptase kit (Qiagen), following the Quick‐Start protocol (Qiagen). ..

    Article Title: Injectable Thermosensitive Hydrogel Containing Bakuchiol Reduces Periodontal Inflammation and Alveolar Bone Loss in a Rat Model
    Article Snippet: For analysis of inflammatory cytokines, gingival tissue around the second molar was biopsied immediately after sacrifice and placed in TRIzol solution (Invitrogen, Carlsbad, CA, USA). .. The tissue was then homogenized using a BioMasher II homogenizer (Nippi, Tokyo, Japan) for several minutes. cDNA was synthesized using the Omniscript Reverse Transcriptase Kit (Qiagen, Germantown, MD, USA), and 10 pmol of each primer ( ) was mixed with Greenstar qRT-PCR Master Mix (Bioneer). .. Real-time PCR analysis was performed using the Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer) to quantify IL-1β gene expression; GAPDH mRNA was used for normalization.

    Article Title: Taurine as a Protective Metabolite in Radiation-Induced Liver Disease: Evidence from 1 H NMR Metabolomics
    Article Snippet: .. Total RNA was isolated using Trizol reagent (Invitrogen) and then reverse transcribed into cDNA using an Omniscript reverse transcriptase kit (Qiagen, Hilden, Germany) in accordance with the manufacturer’s protocol. .. The gene expression level in liver lobes was analyzed using a LightCycler 480 SYBR Green I Master Mix (Roche, Basel, Switzerland) and a CFX Connect real-time PCR system (Bio-Rad, Hercules, CA).

    Article Title: Novel causative RYR2 indel variant with exon and intron involvement inducing exon 13 skipping in a family exhibiting catecholaminergic polymorphic ventricular tachycardia
    Article Snippet: .. Next, the RNA was reverse-transcribed into cDNA using the Omniscript reverse transcriptase kit (Qiagen, Hilden, Germany) and amplified using Platinum II Hotstartaq DNA polymerase (Thermo Fisher Scientific, Waltham, MA, United States) with specifically designed primers ( ). ..

    Article Title: Early life adversity promotes a milieu in favor of catabolic bone turnover in females: Mycobacterium vaccae NCTC 11659 proofs protective in preclinical studies.
    Article Snippet: FFPE Sections were processed according to the manufacturer’s instructions from the Qiagen RNeasy FFPE RNA Extraction kit. .. Isolated RNA was transcribed into cDNA using the Omniscript Reverse Transcriptase Kit (Qiagen, Hilden, Germany). .. Quantitative PCR was performed using the Brilliant SYBR Green QPCR Master Mix Kit (Stratagene, Amsterdam, Netherlands) according to the manufacturer’s protocol in a total volume of 25μl.

    Article Title: UPR/ATF4/Noxa pathway overactivation through SERCA2 inhibition or ONC201 treatment combined with ABT-737 triggers apoptosis in chemoresistant ovarian cancer cells and patient-derived tumor organoids.
    Article Snippet: RNA extraction, Reverse Transcription (RT) and quantitative PCR Total RNAs were isolated using Trizol (Invitrogen, Life Technologies) and quantified using the NanoDropTM 2000 spectrophotometer (ThermoScientific). .. RT was realized with the Omniscript reverse transcriptase kit (Qiagen) with random hexamers on a Mastercycler X40 (Eppendorf). .. For Taq-Man qPCR, cDNA was combined with forward and reverse primers, 1X of the Taq-Man® probe and Taq-Man® Fast Universal PCR Master Mix (Applied Biosystems).

    Article Title: Ectopic Wnt6 expression induces twin-spot markings in the epidermis of the silkworm Bombyx mori - Potential molecular target to discover evolutionary mechanisms of adaptive body coloration in insects.
    Article Snippet: Correspondence J. Zhao and R. Zhang, The Key Laboratory of Aerospace Medicine, Ministry of Education, Fourth Military Medical University, Xi’an, Shaanxi, 710032, China Tel: +86-13310972817 (J. Zhao), +8617802921216 (R. Zhang) E-mail: zhangjd85@fmmu.edu.cn, zhangru@ fmmu.edu.cn F. Jiang, Section of Health, No. 94804 Unit of the Chinese People’s Liberation Army, Shanghai, 200434, China Tel: +86-18189231753 E-mail: fmmujf@chd.edu.cn

    Synthesized:

    Article Title: Injectable Thermosensitive Hydrogel Containing Bakuchiol Reduces Periodontal Inflammation and Alveolar Bone Loss in a Rat Model
    Article Snippet: For analysis of inflammatory cytokines, gingival tissue around the second molar was biopsied immediately after sacrifice and placed in TRIzol solution (Invitrogen, Carlsbad, CA, USA). .. The tissue was then homogenized using a BioMasher II homogenizer (Nippi, Tokyo, Japan) for several minutes. cDNA was synthesized using the Omniscript Reverse Transcriptase Kit (Qiagen, Germantown, MD, USA), and 10 pmol of each primer ( ) was mixed with Greenstar qRT-PCR Master Mix (Bioneer). .. Real-time PCR analysis was performed using the Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer) to quantify IL-1β gene expression; GAPDH mRNA was used for normalization.

    Quantitative RT-PCR:

    Article Title: Injectable Thermosensitive Hydrogel Containing Bakuchiol Reduces Periodontal Inflammation and Alveolar Bone Loss in a Rat Model
    Article Snippet: For analysis of inflammatory cytokines, gingival tissue around the second molar was biopsied immediately after sacrifice and placed in TRIzol solution (Invitrogen, Carlsbad, CA, USA). .. The tissue was then homogenized using a BioMasher II homogenizer (Nippi, Tokyo, Japan) for several minutes. cDNA was synthesized using the Omniscript Reverse Transcriptase Kit (Qiagen, Germantown, MD, USA), and 10 pmol of each primer ( ) was mixed with Greenstar qRT-PCR Master Mix (Bioneer). .. Real-time PCR analysis was performed using the Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer) to quantify IL-1β gene expression; GAPDH mRNA was used for normalization.

    Isolation:

    Article Title: Taurine as a Protective Metabolite in Radiation-Induced Liver Disease: Evidence from 1 H NMR Metabolomics
    Article Snippet: .. Total RNA was isolated using Trizol reagent (Invitrogen) and then reverse transcribed into cDNA using an Omniscript reverse transcriptase kit (Qiagen, Hilden, Germany) in accordance with the manufacturer’s protocol. .. The gene expression level in liver lobes was analyzed using a LightCycler 480 SYBR Green I Master Mix (Roche, Basel, Switzerland) and a CFX Connect real-time PCR system (Bio-Rad, Hercules, CA).

    Article Title: Early life adversity promotes a milieu in favor of catabolic bone turnover in females: Mycobacterium vaccae NCTC 11659 proofs protective in preclinical studies.
    Article Snippet: FFPE Sections were processed according to the manufacturer’s instructions from the Qiagen RNeasy FFPE RNA Extraction kit. .. Isolated RNA was transcribed into cDNA using the Omniscript Reverse Transcriptase Kit (Qiagen, Hilden, Germany). .. Quantitative PCR was performed using the Brilliant SYBR Green QPCR Master Mix Kit (Stratagene, Amsterdam, Netherlands) according to the manufacturer’s protocol in a total volume of 25μl.

    Amplification:

    Article Title: Novel causative RYR2 indel variant with exon and intron involvement inducing exon 13 skipping in a family exhibiting catecholaminergic polymorphic ventricular tachycardia
    Article Snippet: .. Next, the RNA was reverse-transcribed into cDNA using the Omniscript reverse transcriptase kit (Qiagen, Hilden, Germany) and amplified using Platinum II Hotstartaq DNA polymerase (Thermo Fisher Scientific, Waltham, MA, United States) with specifically designed primers ( ). ..

    cDNA Synthesis:

    Article Title: Ectopic Wnt6 expression induces twin-spot markings in the epidermis of the silkworm Bombyx mori - Potential molecular target to discover evolutionary mechanisms of adaptive body coloration in insects.
    Article Snippet: Correspondence J. Zhao and R. Zhang, The Key Laboratory of Aerospace Medicine, Ministry of Education, Fourth Military Medical University, Xi’an, Shaanxi, 710032, China Tel: +86-13310972817 (J. Zhao), +8617802921216 (R. Zhang) E-mail: zhangjd85@fmmu.edu.cn, zhangru@ fmmu.edu.cn F. Jiang, Section of Health, No. 94804 Unit of the Chinese People’s Liberation Army, Shanghai, 200434, China Tel: +86-18189231753 E-mail: fmmujf@chd.edu.cn



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